Open AccessDetermination of DNA sequence changes induced by ethyl methanesulfonate in human cells, using a shuttle vector system.
Author(s) -
Jane Lebkowski,
J.H. Miller,
Michèle P. Calos
Publication year - 1986
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.6.5.1838
Subject(s) - shuttle vector , ethyl methanesulfonate , biology , methyl methanesulfonate , dna , microbiology and biotechnology , mutation , sequence (biology) , dna repair , gene , vector (molecular biology) , genetics , recombinant dna
The DNA sequence changes for 54 mutations induced in human cells by the alkylating agent ethyl methanesulfonate are reported. The mutations were obtained by using a shuttle vector system with the bacterial lacI gene as the target. Of the 54 mutations obtained, 53 were G:C to A:T transitions.