Open AccessMultiple protein-binding sites in the 5'-flanking region regulate c-fos expression.
Author(s) -
Michael Gilman,
Ruth N. Wilson,
Robert A. Weinberg
Publication year - 1986
Publication title -
molecular and cellular biology (print)
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.6.12.4305
Subject(s) - biology , transcription factor , dna , microbiology and biotechnology , transcription (linguistics) , 5' flanking region , gene , gene expression , regulatory sequence , binding site , nuclear protein , promoter , genetics , linguistics , philosophy
We tested sequences flanking the mouse c-fos gene for the ability to form specific DNA-protein complexes with factors present in crude nuclear extracts prepared from mammalian cells. Three such complexes were detected. One complex formed in a region necessary for the induction of c-fos expression by serum growth factors. Two additional complexes formed at sequences that contribute to basal c-fos promoter activity in vivo. These complexes represent three novel sequence-specific DNA-binding activities which appear to participate in the regulation of c-fos transcription.