A nuclear gene of Saccharomyces cerevisiae needed for stable maintenance of plasmids. | Zendy

Yo Kikuchi | Zendy; Akio Tohe | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A nuclear gene of Saccharomyces cerevisiae needed for stable maintenance of plasmids.

Author(s) -

Yo Kikuchi,

Akio Tohe

Publication year - 1986

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.6.11.4053

Subject(s) - biology , plasmid , complementation , saccharomyces cerevisiae , autonomously replicating sequence , genetics , mutant , origin of replication , dna replication , gene , t dna binary system , minichromosome , minichromosome maintenance , microbiology and biotechnology , genome , recombinant dna , vector (molecular biology)

We have isolated host mutants of Saccharomyces cerevisiae in which the 2 microns plasmid is poorly maintained. All the mutants tested constituted one complementation group, which was designated map1 (maintenance of plasmid). Minichromosomes carrying a chromosomal replication origin and a centromere were affected in the mutants. Two types of hybrid plasmids generated in vivo and in vitro appeared to compensate for the mutations and had DNA regions containing multiple ARS (autonomously replicating sequence) or a set of 2 microns inverted repeat sequences. These results suggested that poor maintenance of plasmids was due to low levels of replication, probably at the initiation of replication.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research