Open AccessOverproduction of protein p53 contributes to simian virus 40-mediated transformation.
Author(s) -
Dan Michalovitz,
Daniel Eliyahu,
Moshe Oren
Publication year - 1986
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.6.10.3531
Subject(s) - biology , transformation (genetics) , plasmid , overproduction , mutant , virus , virology , simian , transfection , microbiology and biotechnology , fibroblast , cell culture , gene , genetics
The possible involvement of p53 overproduction in simian virus 40 (SV40)mediated transformation was studied by using the rat embryo fibroblast focus formation assay. Transformation by wild-type SV40 was enhanced two- to threefold by cotransfection of a plasmid overexpressing mouse p53. More significantly, such a plasmid could partially complement a transformation-defective deletion mutant of SV40. Hence, the ability of SV40 T antigen to induce high p53 levels may indeed be directly relevant to the viral transforming potential.