Open AccessLinear simian virus 40 DNA fragments exhibit a propensity for rolling-circle replication.
Author(s) -
Ida Deichaite,
Z Laver-Rudich,
Dale Dorsett,
Ernest Winocour
Publication year - 1985
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.5.7.1787
Subject(s) - biology , rolling circle replication , dna replication , dna , microbiology and biotechnology , virus , simian , dna supercoil , virology , viral replication , genetics
A linear simian virus 40 origin-containing DNA fragment replicated in monkey COS cells, generating tandemly repeated (head-to-tail) structures. Electron microscopy revealed circle-and-tail configurations characteristic of rolling-circle replication intermediates. Circularization of the same DNA before transfection led to a theta type of replication which generated supercoiled DNA molecules.