Open AccessInduction of cellular DNA synthesis by a simian virus 40 mutant defective in nuclear transport of T antigen.
Author(s) -
Robert E. Lanford,
J K Hyland,
R Baserga,
Janet S. Butel
Publication year - 1985
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.5.6.1531
Subject(s) - biology , microbiology and biotechnology , dna synthesis , mutant , sv40 large t antigen , plasmid , dna , dna replication , proliferating cell nuclear antigen , antigen , cell culture , virus , simian , transfection , virology , gene , genetics
The simian virus 40 (SV40) (cT)-3 mutant [SV40(cT)-3], which is defective in nuclear transport of T antigen, was utilized to determine whether cellular DNA synthesis can be stimulated by SV40 in the absence of detectable nuclear T antigen. Cellular DNA synthesis was examined in the temperature-sensitive cell cycle mutants, BHK ts13 and BHK tsAF8, after microinjection of quiescent cells with plasmid DNA containing cloned copies of wild-type SV40 or SV40(cT)-3. The efficiency of induction of cellular DNA synthesis was identical for both wild-type SV40 and SV40(cT)-3 in both cell lines. The results suggest that cell surface-associated T antigen, either alone or possibly in combination with minimal amounts of nuclear T antigen below our limit of detection, is able to stimulate cellular DNA synthesis.