Open AccessThe bovine papillomavirus distal "enhancer" is not cis essential for transformation or for plasmid maintenance.
Author(s) -
Peter M. Howley,
E T Schenborn,
E Lund,
J C Byrne,
J E Dahlberg
Publication year - 1985
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.5.11.3310
Subject(s) - biology , enhancer , plasmid , bovine papillomavirus , mutant , transformation (genetics) , microbiology and biotechnology , dna , polyadenylation , gene , genome , genetics , rna , gene expression
We constructed a mutant of bovine papillomavirus type 1 (BPV-1) DNA that lacked a transcriptional enhancer located 3' to the polyadenylation site of the early viral RNAs expressed in transformed cells. This mutant DNA, when separated from the procaryotic sequences, transforms mouse cells with an efficiency comparable to that of the full BPV-1 genome, and it exists as a stable multicopy plasmid in transformed cells. The BPV-1 distal enhancer suppresses the effects of a cis-inhibitory element in pML2 sequences but is not essential for the expression of the viral genes involved in cellular transformation or plasmid maintenance.