Open AccessDNA-mediated transfer and expression of a human DNA repair gene that demethylates O6-methylguanine.
Author(s) -
R Ding,
K Ghosh,
Alan Eastman,
Edward Bresnick
Publication year - 1985
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.5.11.3293
Subject(s) - biology , microbiology and biotechnology , dna , transfection , gene , dna repair , o 6 methylguanine dna methyltransferase , genetics , methyltransferase , methylation
Human liver DNA was transfected into CHO cells (mex-) along with pSV2gpt and colonies were selected first for resistance to mycophenolic acid and then to chloroethylnitrosourea. Transformants were obtained that contained approximately 10,000 molecules of O6-alkylguanine alkyltransferase (mex+) per cell. Their genome contained at least three copies of the human Alu sequence.