Stable transcription complex on a class III gene in a minichromosome. | Zendy

Andrew B. Lassar | Zendy; Dean H. Hamer | Zendy; Robert G. Roeder | Zendy

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Stable transcription complex on a class III gene in a minichromosome.

Author(s) -

Andrew B. Lassar,

Dean H. Hamer,

Robert G. Roeder

Publication year - 1985

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.5.1.40

Subject(s) - biology , minichromosome , rna polymerase ii , transcription (linguistics) , transcription factor ii d , microbiology and biotechnology , rna polymerase i , transcription factories , rna polymerase , rna , rna dependent rna polymerase , polymerase , general transcription factor , gene , chromatin , gene expression , genetics , promoter , linguistics , philosophy

We have constructed recombinant simian virus 40 molecules containing Xenopus 5S RNA and tRNA genes. Recombinant minichromosomes containing these genes were isolated to study the interaction of RNA polymerase III transcription factors with these model chromatin templates. Minichromosomes containing a tRNAMet gene can be isolated in a stable complex with transcription factors (IIIB and IIIC) and are active in vitro templates for purified RNA polymerase III. In contrast, minichromosomes containing a 5S RNA gene are refractory to transcription by purified RNA polymerase III in either the absence or the presence of other factors.

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