Open AccessDirect isolation of neuronal microtubule skeletons.
Author(s) -
Gary W. Zieve,
F Solomon
Publication year - 1984
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.4.2.371
Subject(s) - microtubule , biology , microbiology and biotechnology , tubulin , microtubule associated protein , isolation (microbiology) , computational biology , bioinformatics
The microtubule skeletons of cultured neuronal cells have been isolated in their original form utilizing taxol and rigorous extraction conditions. Continuous microtubules are visible throughout the cell body and into the processes. These preparations include the complex set of microtubule-associated proteins of 69,000 and 80,000 daltons previously identified in these cells.
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