Open AccessSequence organization of a pea chloroplast DNA gene coding for a 34,500-dalton protein.
Author(s) -
K K Oishi,
Daniel R. Shapiro,
Κ. Κ. Tewari
Publication year - 1984
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.4.11.2556
Subject(s) - biology , chloroplast dna , gene , coding region , microbiology and biotechnology , promoter , dna , genetics , spinach , transcription (linguistics) , homology (biology) , nucleic acid sequence , chloroplast , gene expression , biochemistry , linguistics , philosophy
A gene (PGII), which codes for a 34.5-kilodalton protein, has been isolated and cloned from pea chloroplast DNA. The production of its 1.2-kilobase mRNA is photodependent. The direction of transcription has been determined, the site of initiation of transcription has been found, and an in vitro protein product has been produced. The gene, including the 5' and 3'-flanking regions, has been sequenced. It shows ca. 95% homology to the photosystem II thylakoid membrane protein, photogene 32, from spinach and tobacco. There are no intervening sequences. The 5'-flanking region suggests similarities with Escherichia coli promoters. The 5'-flanking region is remarkably conserved among pea, spinach, and tobacco DNA.