Open AccessError-prone mutagenesis detected in mammalian cells by a shuttle vector containing the supF gene of Escherichia coli.
Author(s) -
Saumyen N. Sarkar,
Uma B. Dasgupta,
William C. Summers
Publication year - 1984
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.4.10.2227
Subject(s) - shuttle vector , biology , mutagenesis , microbiology and biotechnology , mutation frequency , transfection , mutation , ethyl methanesulfonate , vector (molecular biology) , cell culture , gene , genetics , recombinant dna
When a shuttle vector containing a tyrosine suppressor tRNA (supF) gene as a target for mutagenesis replicated in a monkey kidney cell line, the frequency of SupF+ mutations was 2.3 +/- 0.5 x 10(-3). When the host cells were treated with ethyl methanesulfonate 40 h before transfection, a 10-fold increase in SupF+ mutation frequency was observed. These results supported the hypothesis that a damage-inducible mutagenic pathway exists in mammalian cells and also demonstrated the utility of this shuttle vector for the study of mutagenesis in mammalian cells.