Open AccessAntibody-induced modulation of proteins in vesicular stomatitis virus-infected fibroblasts.
Author(s) -
E J O'Rourke,
W H Guo,
Alice S. Huang
Publication year - 1983
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.3.9.1580
Subject(s) - vesicular stomatitis virus , biology , vesicular stomatitis , virus , vesicular stomatitis indiana virus , baby hamster kidney cell , virology , cytochalasin b , glycoprotein , viral envelope , cytochalasin , antiserum , antibody , cytopathic effect , rhabdoviridae , microbiology and biotechnology , biochemistry , cell , immunology , cytoskeleton
When vesicular stomatitis virus-infected baby hamster kidney cells were treated with rabbit anti-vesicular stomatitis virus serum, there was a loss of the viral glycoprotein G into acid-soluble products. This degradation occurred within minutes at 37 degrees C and required the presence of G protein at the cell surface. The degree of degradation depended on antiserum concentration. The antiserum, also, prevented maturation of extracellular virions and induced partial degradation of the intracellular viral proteins, without affecting host proteins. The degradation could not be prevented by the presence of lysosomotropic agents, protease inhibitors, colchicine, or cytochalasin B. Similar kinetics and specificity of degradation was obtained with cells infected with vesicular stomatitis virus mutants that were less cytopathic. These results characterize a model system for studying the parameters and consequences of antigenic modulation as well as for studying the fate of viral antigens during persistent infections.