Open AccessMultiple, Tandem Plasmid Integration inSaccharomyces cerevisiae
Author(s) -
Terry L. OrrWeaver,
Jack W. Szostak
Publication year - 1983
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.3.4.747
Subject(s) - plasmid , saccharomyces cerevisiae , biology , t dna binary system , genetics , tandem , transformation (genetics) , restriction enzyme , tandem repeat , genome , computational biology , restriction map , dna , recombinant dna , gene , vector (molecular biology) , materials science , composite material
Nonreplicating plasmids transform Saccharomyces cerevisiae by recombining with a homologous site in the genome. Frequently, multiple copies of the plasmid integrate in a tandem array. We show that, after transformation with restriction enzyme-cut plasmids, most, if not all, multimers arise by sequential integration of plasmid molecules into the same genomic location.