Open AccessMultiple Roles of Vertebrate REV Genes in DNA Repair and Recombination
Author(s) -
Takashi Okada,
Eiichiro Sonoda,
Masataka Yoshimura,
Yoshiaki Kawano,
Hideyuki Saya,
Masaoki Kohzaki,
Shunichi Takeda
Publication year - 2005
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.25.14.6103-6111.2005
Subject(s) - biology , homologous recombination , mutagenesis , dna damage , mutant , dna repair , somatic hypermutation , genetics , mutation , dna , non homologous end joining , gene conversion , dna repair protein xrcc4 , microbiology and biotechnology , mitotic crossover , gene , recombination , b cell , nucleotide excision repair , antibody
In yeast, Rev1, Rev3, and Rev7 are involved in translesion synthesis over various kinds of DNA damage and spontaneous and UV-induced mutagenesis. Here, we disrupted Rev1, Rev3, and Rev7 in the chicken B-lymphocyte line DT40. REV1-/- REV3-/- REV7-/- cells showed spontaneous cell death, chromosomal instability/fragility, and hypersensitivity to various genotoxic treatments as observed in each of the single mutants. Surprisingly, the triple-knockout cells showed a suppressed level of sister chromatid exchanges (SCEs), which may reflect postreplication repair events mediated by homologous recombination, while each single mutant showed an elevated SCE level. Furthermore, REV1-/- cells as well as triple mutants showed a decreased level of immunoglobulin gene conversion, suggesting participation of Rev1 in a recombination-based pathway. The present study gives us a new insight into cooperative function of three Rev molecules and the Polzeta (Rev3-Rev7)-independent role of Rev1 in vertebrate cells.