Open AccessARA67/PAT1 Functions as a Repressor To Suppress Androgen Receptor Transactivation
Author(s) -
Yanqing Zhang,
Yue Yang,
Shuyuan Yeh,
Chawnshang Chang
Publication year - 2004
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.24.3.1044-1057.2004
Subject(s) - transactivation , biology , androgen receptor , repressor , prostate cancer , corepressor , nuclear receptor , receptor , glucocorticoid receptor , microbiology and biotechnology , cancer research , transcription factor , genetics , cancer , gene
The androgen receptor (AR) may recruit multiple coregulators for proper or optimal transactivation. Here we report the identification and characterization of ARA67/PAT1 as an AR coregulator from a prostate cDNA library. ARA67/PAT1 was screened out as an AR N terminus interacting protein. Interaction mapping shows that the cooperation of multiple domains within ARA67/PAT1 may be required for the maximal interaction with AR. ARA67/PAT1 functions as a repressor with better suppressive effects on AR compared to glucocorticoid receptor and estrogen receptor. Further mechanism dissection reveals that the interrupted AR cytoplasmic-nuclear shuttling may play a major role in ARA67/PAT1 mediated suppression on AR. Together, these results suggest that ARA67/PAT1 may function as a novel repressor that can modulate AR function in prostate cancer.