p53 Mutants Have Selective Dominant-Negative Effects on Apoptosis but Not Growth Arrest in Human Cancer Cell Lines | Zendy

Oscar Aurelio | Zendy; Xiao–Tang Kong | Zendy; Swati Gupta | Zendy; Eric J. Stanbridge | Zendy

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p53 Mutants Have Selective Dominant-Negative Effects on Apoptosis but Not Growth Arrest in Human Cancer Cell Lines

Author(s) -

Oscar Aurelio,

Xiao–Tang Kong,

Swati Gupta,

Eric J. Stanbridge

Publication year - 2000

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.20.3.770-778.2000

Subject(s) - transactivation , biology , mutant , transfection , apoptosis , wild type , cancer research , microbiology and biotechnology , cell cycle checkpoint , cell culture , expression vector , cell cycle , transcription factor , gene , genetics , recombinant dna

A bidirectional expression vector that allowed equal transcription of cloned wild-type and mutant p53 cDNAs from the same vector was developed. The vector was transfected into CaLu 6 lung carcinoma cells or Saos-2 osteosarcoma cells. All p53 mutants examined were recessive to wild-type p53 transactivation ofp21WAF1/CIP1 but dominant-negative for transactivation ofBax . An examination of effects on growth arrest and apoptotic pathways indicated that all mutants were recessive to wild type for growth arrest but only three of seven mutants were dominant negative for induction of apoptosis.

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