Open AccessRegulatory Networks Revealed by Transcriptional Profiling of DamagedSaccharomyces cerevisiaeCells: Rpn4 Links Base Excision Repair with Proteasomes
Author(s) -
Scott A. Jelinsky,
Preston W. Estep,
George M. Church,
Leona D. Samson
Publication year - 2000
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.20.21.8157-8167.2000
Subject(s) - biology , gene , saccharomyces cerevisiae , genetics , dna repair , transcription factor , proteasome , regulatory sequence
Exposure to carcinogenic alkylating agents, oxidizing agents, and ionizing radiation modulates transcript levels for over one third ofSaccharomyces cerevisiae's 6,200 genes. Computational analysis delineates groups of coregulated genes whose upstream regions bear known and novel regulatory sequence motifs. One group of coregulated genes contain a number of DNA excision repair genes (including theMAG1 3-methyladenine DNA glycosylase gene) and a large selection of protein degradation genes. Moreover, transcription of these genes is modulated by the proteasome-associated protein Rpn4, most likely via its binding toMAG1 upstream repressor sequence 2-like elements, that turn out to be almost identical to the recently identified proteasome-associated control element (G. Mannhaupt, R. Schnall, V. Karpov, I. Vetter, and H. Feldmann, FEBS Lett. 450:27–34, 1999). We have identified a large number of genes whose transcription is influenced by Rpn4p.