Open AccessNuclear Inheritance of Erythromycin Resistance in Human Cells: New Class of Mitochondrial Protein Synthesis Mutants
Author(s) -
Claus-Jens Doersen,
Eric J. Stanbridge
Publication year - 1982
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.2.6.694
Subject(s) - biology , mutant , mutation , genetics , erythromycin , phenotype , microbiology and biotechnology , mitochondrial dna , hela , gene , cell culture , antibiotics
The characterization of two new erythromycin-resistant mutants of HeLa cells is described. The strains ERY2305 and ERY2309 both exhibited resistance to erythromycin in growth assays and cell-free mitochondrial protein synthesis assays. The erythromycin resistance phenotype could not be transferred by cybridization. The mutation appeared to be encoded in the nucleus and inherited as a recessive trait. These two mutants, therefore, represent a new class of erythromycin-resistant mutants in human cells that is distinct from the cytoplasmically inherited mutation in strain ERY2301 described previously.