Open Accessc-Maf Interacts with c-Myb To Regulate Transcription of an Early Myeloid Gene during Differentiation
Author(s) -
Shrikanth P. Hegde,
Ashish Kumar,
Cornelia Kurschner,
Linda H. Shapiro
Publication year - 1998
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.18.5.2729
Subject(s) - biology , transactivation , myeloid , myb , transcription factor , haematopoiesis , gene expression , regulation of gene expression , microbiology and biotechnology , transcription (linguistics) , activator (genetics) , gene , cancer research , stem cell , genetics , linguistics , philosophy
The MafB transcriptional activator plays a pivotal role in regulating lineage-specific gene expression during hematopoiesis by repressing Ets-1-mediated transcription of key erythroid-specific genes in myeloid cells. To determine the effects of Maf family proteins on the transactivation of myeloid-specific genes in myeloid cells, we tested the ability of c-Maf to influence Ets-1- and c-Myb-dependentCD13/APN transcription. Expression of c-Maf in human immature myeloblastic cells inhibitedCD13/APN -driven reporter gene activity (85 to 95% reduction) and required the binding of both c-Myb and Ets, but not Maf, to the promoter fragment. c-Maf’s inhibition ofCD13/APN expression correlates with its ability to physically associate with c-Myb. While c-Maf mRNA and protein levels remain constant during myeloid differentiation, formation of inhibitory Myb-Maf complexes was developmentally regulated, with their levels being highest in immature myeloid cell lines and markedly decreased in cell lines representing later developmental stages. This pattern matched that ofCD13/APN reporter gene expression, indicating that Maf modulation of c-Myb activity may be an important mechanism for the control of gene transcription during hematopoietic cell development.