Integration of an Insertion-Type Transferred DNA Vector from Agrobacterium tumefaciens into the Saccharomyces cerevisiae Genome by Gap Repair | Zendy

Eddy Risseeuw | Zendy; Marry E.I. Frankevan Dijk | Zendy; Paul J. J. Hooykaas | Zendy

Open Access

Integration of an Insertion-Type Transferred DNA Vector from Agrobacterium tumefaciens into the Saccharomyces cerevisiae Genome by Gap Repair

Author(s) -

Eddy Risseeuw,

Marry E.I. Frankevan Dijk,

Paul J. J. Hooykaas

Publication year - 1996

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.16.10.5924

Subject(s) - biology , saccharomyces cerevisiae , homologous recombination , agrobacterium tumefaciens , genetics , dna , shuttle vector , insert (composites) , transfer dna , transformation (genetics) , flp frt recombination , dna repair , in vitro recombination , agrobacterium , recombination , genetic recombination , vector (molecular biology) , yeast , gene , molecular cloning , recombinant dna , peptide sequence , mechanical engineering , engineering

Recently, it was shown that Agrobacterium tumefaciens can transfer transferred DNA (T-DNA) to Saccharomyces cerevisiae and that this T-DNA, when used as a replacement vector, is integrated via homologous recombination into the yeast genome. To test whether T-DNA can be a suitable substrate for integration via the gap repair mechanism as well, a model system developed for detection of homologous recombination events in plants was transferred to S. cerevisiae. Analysis of the yeast transformants revealed that an insertion type T-DNA vector can indeed be integrated via gap repair. Interestingly, the transformation frequency and the type of recombination events turned out to depend strongly on the orientation of the insert between the borders in such an insertion type T-DNA vector.

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