Open AccessMultiple cis-acting targeting sequences are required for orb mRNA localization during Drosophila oogenesis.
Author(s) -
Valerie Lantz,
Paul Schedl
Publication year - 1994
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.14.4.2235
Subject(s) - biology , oocyte , untranslated region , orb (optics) , microbiology and biotechnology , messenger rna , three prime untranslated region , oogenesis , drosophila (subgenus) , genetics , drosophila embryogenesis , drosophila melanogaster , embryo , gene , artificial intelligence , computer science , image (mathematics)
The targeting of positional information to specific regions of the oocyte or early embryo is one of the key processes in establishing anterior-posterior and dorsal-ventral polarity. In many developmental systems, this is accomplished by localization of mRNAs. The germ line-specific Drosophila orb gene plays a critical role in defining both axes of the developing oocyte, and its mRNA is localized in a complex pattern during oogenesis. We have identified a 280-bp sequence from the orb 3' untranslated region capable of reproducing this complex localization pattern. Furthermore, we have found that multiple cis-acting elements appear to be required for proper targeting of orb mRNA.