Open AccessTATA-binding protein and associated factors in polymerase II and polymerase III transcription.
Author(s) -
R E Meyers,
P A Sharp
Publication year - 1993
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.13.12.7953
Subject(s) - transcription factor ii d , transcription factor ii a , rna polymerase iii , transcription factor ii b , tata binding protein , general transcription factor , biology , transcription factor ii f , rna polymerase ii , taf2 , tata box binding protein , taf1 , microbiology and biotechnology , rna polymerase ii holoenzyme , transcription factor , polymerase , genetics , dna binding protein , promoter , transcriptional regulation , dna , gene expression , gene , rna dependent rna polymerase , enhancer
Transcription by RNA polymerase I (pol I), pol II, and pol III requires the TATA-binding protein (TBP). This protein functions in association with distinct TBP-associated factors (TAFs) which may specify the nature of the polymerase selected for initiation at a promoter site. In the pol III transcription system, the TBP-TAF complex is a component of the TFIIIB factor. This factor has been resolved into a TBP-TAF complex and another component, both of which are required for reconstitution of transcription by pol III. Neither the TBP-TAF complexes B-TFIID and D-TFIID, which were previously characterized as active for pol II transcription, nor TBP alone can complement pol III transcription reactions that are dependent upon the TBP-TAF subcomponent of TFIIIB. Surprisingly, the TBP-TAF subcomponent of TFIIIB is active in reconstitution of pol II transcription.