Open AccessYin-yang 1 activates the c-myc promoter.
Author(s) -
K J Riggs,
Shireen Saleque,
Kwok-Kin Wong,
Kevin Merrell,
Jeng-Shin Lee,
Yang Shi,
Kathryn Calame
Publication year - 1993
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.13.12.7487
Subject(s) - biology , microbiology and biotechnology , promoter , zinc finger , activator (genetics) , transcription factor , transcription (linguistics) , yy1 , recombinant dna , gene , binding site , sp1 transcription factor , gene expression , genetics , linguistics , philosophy
Previous studies on the murine c-myc promoter demonstrated that a ubiquitously present protein, common factor 1 (CF1), bound at two sites located -260 and -390 bp from the P1 transcription start site. CF1 has been purified to near homogeneity and shown to be identical to the zinc finger protein Yin-yang 1 (YY1) as judged by similarity of molecular weight and other biochemical properties, immunological cross-reactivity, and the ability of recombinant YY1 to bind to CF1 sites. In cotransfection experiments, YY1 is a strong activator of transcription from c-myc promoter-based reporters. Furthermore, in murine erythroleukemia cells, overexpressed YY1 causes increased levels of c-myc mRNA initiated from both major transcription initiation sites of the endogenous c-myc gene.