Open AccessStructure of the chicken myelomonocytic growth factor gene and specific activation of its promoter in avian myelomonocytic cells by protein kinases.
Author(s) -
E Sterneck,
C Blattner,
T Graf,
Achim Leutz
Publication year - 1992
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.12.4.1728
Subject(s) - biology , microbiology and biotechnology , acute myelomonocytic leukemia , runx1t1 , interleukin 3 , kinase , gene , autocrine signalling , chronic myelomonocytic leukemia , myeloid , cancer research , gene expression , leukemia , cell culture , genetics , immunology , t cell , bone marrow , myelodysplastic syndromes , immune system , antigen presenting cell
In chicken myeloid cells but not in erythroid cells, kinase-type oncogenes activate expression of the chicken myelomonocytic growth factor (cMGF). The autocrine loop established this way plays a key role in lineage-specific cooperation of nuclear and kinase-type oncogenes in retrovirally induced myeloid leukemia. In this report, we describe the cloning of the cMGF gene, including its promoter. The structure of the cMGF gene is homologous to those of the granulocyte colony-stimulating factor and interleukin-6 genes. Expression from reporter constructs containing the cMGF promoter is specific to myelomonocytic cells. Kinases activate cMGF at the transcriptional level in macrophages and strongly induce reporter expression in myelomonocytic cells.