Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene. | Zendy

Stephen D. Lupton | Zendy; L L Brunton | Zendy; V A Kalberg | Zendy; Robert W. Overell | Zendy

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Dominant positive and negative selection using a hygromycin phosphotransferase-thymidine kinase fusion gene.

Author(s) -

Stephen D. Lupton,

L L Brunton,

V A Kalberg,

Robert W. Overell

Publication year - 1991

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.11.6.3374

Subject(s) - biology , thymidine kinase , hygromycin b , fusion gene , gene , microbiology and biotechnology , negative selection , genetics , virology , selectable marker , phosphotransferase , herpes simplex virus , plasmid , virus , genome

The hygromycin phosphotransferase gene was fused in-frame with the herpes simplex virus type 1 thymidine kinase gene. The resulting fusion gene (termed HyTK) confers hygromycin B resistance for dominant positive selection and ganciclovir sensitivity for negative selection and provides a means by which these selectable phenotypes may be expressed and regulated as a single genetic entity.

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