Open AccessThe Saccharomyces cerevisiae SRK1 gene, a suppressor of bcy1 and ins1, may be involved in protein phosphatase function.
Author(s) -
Robert B. Wilson,
Alfred Brenner,
T B White,
Michael Engler,
J P Gaughran,
Kelly Tatchell
Publication year - 1991
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.11.6.3369
Subject(s) - biology , saccharomyces cerevisiae , schizosaccharomyces pombe , cyclin dependent kinase 7 , mutant , phosphatase , protein kinase a , shuttle vector , gene , microbiology and biotechnology , biochemistry , kinase , enzyme , mitogen activated protein kinase kinase , recombinant dna , vector (molecular biology)
The Saccharomyces cerevisiae SRK1 gene, when expressed on a low-copy shuttle vector, partially suppresses the phenotype associated with elevated levels of cyclic AMP-dependent protein kinase activity and suppresses the temperature-sensitive cell cycle arrest of the ins1 mutant. SRK1 is located on chromosome IV, 3 centimorgans from gcn2. A mutant carrying a deletion mutation in srk1 is viable. SRK1 encodes a 140-kDa protein with homology to the dis3+ protein from Schizosaccharomyces pombe. The ability of SRK1 to alleviate partially the defects caused by high levels of cyclic AMP-dependent protein kinase and the similarity of its encoded protein to dis3+ suggest that SRK1 may have a role in protein phosphatase function.