Open AccessCa2+ is essential for multistep activation of the heat shock factor in permeabilized cells.
Author(s) -
Brendan D. Price,
Stuart K. Calderwood
Publication year - 1991
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.11.6.3365
Subject(s) - heat shock factor , biology , hsp70 , transcription factor , hsf1 , heat shock , microbiology and biotechnology , heat shock protein , transcription (linguistics) , biophysics , gene , biochemistry , linguistics , philosophy
We have developed a novel permeabilized-cell system to study transcription mechanisms. In permeabilized cells, heat-induced activation of the heat shock factor and transcription of the hsp70 gene require Ca2+. Activation involves at least two steps: Ca(2+)- and heat-dependent activation of heat shock factor binding and a second step, prior to transcription of hsp70, that requires ATP and is sensitive to genistein, a protein kinase inhibitor.