Cross-resistance to UV radiation of a cisplatin-resistant human cell line: overexpression of cellular factors that recognize UV-modified DNA. | Zendy

ChihYing Chao | Zendy; Shuxin Huang | Zendy; Hongbiao Huang | Zendy; Sue LinChao | Zendy

Open Access

Cross-resistance to UV radiation of a cisplatin-resistant human cell line: overexpression of cellular factors that recognize UV-modified DNA.

Author(s) -

ChihYing Chao,

Shuxin Huang,

Hongbiao Huang,

Sue LinChao

Publication year - 1991

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.11.4.2075

Subject(s) - biology , chloramphenicol acetyltransferase , cisplatin , transfection , dna , cell culture , microbiology and biotechnology , dna repair , plasmid , dna damage , gene , biochemistry , reporter gene , genetics , gene expression , chemotherapy

A human cell line selected for cisplatin resistance (CPR) was irradiated with UV light and showed cross-resistance to UV light. Applying a modified chloramphenicol acetyltransferase assay, we observed that CPR cells acquired enhanced host cell reactivation of a transfected plasmid carrying UV damage. Gel mobility shift analysis indicated that two nuclear factors that recognize UV-modified DNA were overexpressed in CPR cells. In addition, factors that bind UV-modified DNA were independent from the factors that bind cisplatin-modified DNA. The significance of the identified binding factors, possibly DNA repair enzymes, is discussed.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research