Open AccessThe zinc finger protein GLI transforms primary cells in cooperation with adenovirus E1A.
Author(s) -
J. Michael Ruppert,
Bert Vogelstein,
Kenneth W. Kinzler
Publication year - 1991
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.11.3.1724
Subject(s) - biology , zinc finger , microbiology and biotechnology , mastadenovirus , adenoviridae , transformation (genetics) , in vitro , cancer research , gene , genetics , genetic enhancement , transcription factor
The GLI gene was previously isolated by virtue of its amplification in human glioblastomas. We have now found that GLI expression can result in the in vitro transformation of both primary and secondary rodent cells. When coexpressed with adenovirus E1A, the GLI protein functions analogously to RAS, resulting in the formation of dense foci of cells which are tumorigenic in nude mice.