Open AccessMeiotic induction by Xenopus cyclin B is accelerated by coexpression with mosXe.
Author(s) -
Robert S. Freeman,
Scott Ballantyne,
Daniel J. Donoghue
Publication year - 1991
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.11.3.1713
Subject(s) - biology , cyclin b , xenopus , cyclin a2 , cyclin , germinal vesicle , cyclin a , cyclin b1 , cyclin d , microinjection , cyclin e , microbiology and biotechnology , cyclin e1 , cyclin dependent kinase complex , cell cycle , oocyte , cyclin dependent kinase 1 , genetics , gene , embryo
We have investigated the relationship between Xenopus laevis c-mos (mosXe) and the cyclin B component of maturation-promoting factor. Microinjection of Xenopus oocytes with in vitro-synthesized RNAs encoding Xenopus cyclin B1 or cyclin B2 induces the progression of meiosis, characterized by germinal vesicle breakdown (GVBD). By preinjecting oocytes with a mosXe-specific antisense oligonucleotide, we show that GVBD induced by cyclin B does not require expression of the mosXe protein. GVBD induced by cyclin B proceeds significantly faster than GVBD induced by progesterone or MosXe. However, coinjection of RNAs encoding cyclin B1 or cyclin B2 with mosXe RNA results in a 2.5- to 3-fold acceleration in GVBD relative to that induced by cyclin B alone. This acceleration of GVBD does not correlate with changes in the level of cyclin B1 and cyclin B2 phosphorylation.