Open AccessProhibitin, an evolutionarily conserved intracellular protein that blocks DNA synthesis in normal fibroblasts and HeLa cells.
Author(s) -
Mark J. Nuell,
David A. Stewart,
Lucy Walker,
Varda Friedman,
Carla M. Wood,
Garrison Owens,
James R. Smith,
Edward L. Schneider,
R Dell' Orco,
Charles K. Lumpkin
Publication year - 1991
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.11.3.1372
Subject(s) - prohibitin , biology , microinjection , microbiology and biotechnology , hela , complementary dna , oligonucleotide , gene , cell culture , mitochondrion , genetics
Genes that act inside the cell to negatively regulate proliferation are of great interest because of their implications for such processes as development and cancer, but these genes have been difficult to clone. This report details the cloning and analysis of cDNA for prohibitin, a novel mammalian antiproliferative protein. Microinjection of synthetic prohibitin mRNA blocks entry into S phase in both normal fibroblasts and HeLa cells. Microinjection of an antisense oligonucleotide stimulates entry into S phase. By sequence comparison, the prohibitin gene appears to be the mammalian analog of Cc, a Drosophila gene that is vital for normal development.