The ubiquitous transcription factor Oct-1 and the liver-specific factor HNF-1 are both required to activate transcription of a hepatitis B virus promoter. | Zendy

DaoXiu Zhou | Zendy; T S Yen | Zendy

Open Access

The ubiquitous transcription factor Oct-1 and the liver-specific factor HNF-1 are both required to activate transcription of a hepatitis B virus promoter.

Author(s) -

DaoXiu Zhou,

T S Yen

Publication year - 1991

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.11.3.1353

Subject(s) - biology , promoter , hepatocyte nuclear factors , transcription factor , sp3 transcription factor , transcription (linguistics) , microbiology and biotechnology , tata box , e box , taf2 , response element , general transcription factor , hepatocyte nuclear factor 4 , hepatitis b virus , gene , virology , gene expression , genetics , virus , philosophy , nuclear receptor , linguistics

The liver-specific transcription factor HNF-1 activates transcription of several mammalian hepatocyte-specific genes. The hepatitis B virus preS1 promoter shows hepatocyte specificity, which has been ascribed to binding of HNF-1 to a cognate DNA sequence upstream of the TATA box. We show here that there is an adjacent site that binds the ubiquitous transcription factor Oct-1. Both the Oct-1 and HNF-1 sites are necessary for liver-specific transcription of the preS1 promoter, but neither site alone activates transcription. The Oct-1 site is also necessary for activation of the preS1 promoter in HeLa cells, expressing transfected HNF-1. Our results show that while Oct-1 is not restricted to hepatocytes, it nevertheless can play a critical role in the expression of a liver-specific gene.

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