Transcription complex disruption caused by a transition in chromatin structure. | Zendy

Geneviève Almouzni | Zendy; Marcel Méchali | Zendy; Alan P. Wolffe | Zendy

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Transcription complex disruption caused by a transition in chromatin structure.

Author(s) -

Geneviève Almouzni,

Marcel Méchali,

Alan P. Wolffe

Publication year - 1991

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.11.2.655

Subject(s) - chromatin , biology , xenopus , nucleosome , histone modifying enzymes , transcription (linguistics) , histone , microbiology and biotechnology , chia pet , bivalent chromatin , transcription preinitiation complex , dna , genetics , gene , promoter , gene expression , linguistics , philosophy

Chromatin structure is known to influence class III gene expression in vitro. We describe the active transcription of Xenopus class III genes following replication and assembly into chromatin by using Xenopus egg extracts. Changes in the structure of this active chromatin dependent on the presence of exogeneous Mg2+ ATP or on the addition of a mixture of histones H2A and H2B are shown to lead to the selective repression of Xenopus 5S RNA genes. Preexisting transcription complexes on 5S DNA are disrupted following the reorganization of a "disordered" histone-DNA complex into a structure consisting of physiologically spaced nucleosomes. Thus, we demonstrate that chromatin structural transitions can have dominant and specific effects on transcription.

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