Open AccessInduction of NF-kappa B DNA-binding activity during the G0-to-G1 transition in mouse fibroblasts.
Author(s) -
Albert S. Baldwin,
Jane Clifford Azizkhan,
David E. Jensen,
Amer A. Beg,
Lavanya R. Coodly
Publication year - 1991
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.11.10.4943
Subject(s) - biology , cycloheximide , microbiology and biotechnology , enhancer , 3t3 cells , transition (genetics) , dna , nfkb1 , dna synthesis , dna binding protein , kappa , gene , gene expression , transcription factor , transfection , biochemistry , protein biosynthesis , linguistics , philosophy
A DNA-binding factor with properties of NF-kappa B and another similar activity are rapidly induced when growth-arrested BALB/c 3T3 cells are stimulated with serum growth factors. Induction of these DNA-binding activities is not inhibited by pretreatment of quiescent cells with the protein synthesis inhibitor cycloheximide. Interestingly, the major NF-kappa B-like activity is not detected in nuclear extracts of proliferating cells, and thus its expression appears to be limited to the G0-to-G1 transition in 3T3 cells. These DNA-binding activities bind many of the expected NF-kappa B target sequences, including elements in the class I major histocompatibility complex and human immunodeficiency virus enhancers, as well as a recently identified NF-kappa B binding site upstream of the c-myc gene. Furthermore, both the class I major histocompatibility complex and c-myc NF-kappa B binding sites confer inducibility on a minimal promoter in 3T3 cells stimulated with serum growth factors. The results demonstrate that NF-kappa B-like activities are immediate-early response proteins in 3T3 cells and suggest a role for these factors in the G0-to-G1 transition.