Open AccessInduction of growth arrest by a temperature-sensitive p53 mutant is correlated with increased nuclear localization and decreased stability of the protein.
Author(s) -
Doron Ginsberg,
Dan Michael-Michalovitz,
Moshe Oren
Publication year - 1991
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.11.1.582
Subject(s) - biology , mutant , nuclear protein , protein stability , cytoplasm , cell growth , microbiology and biotechnology , cell nucleus , nuclear localization sequence , p53 protein , apoptosis , biochemistry , gene , transcription factor
A temperature-sensitive mutant of p53, p53Val-135, was found to be able to arrest cell proliferation when overexpressed at 32.5 degrees C. While much of the protein was cytoplasmic in cells proliferating at 37.5 degrees C, it became predominantly nuclear at 32.5 degrees C. Concomitantly, p53Val-135 became destabilized, although not to the extent seen in primary fibroblasts.