Open AccessA chicken beta-actin gene can complement a disruption of the Saccharomyces cerevisiae ACT1 gene.
Author(s) -
Roger Karlsson,
Pontus Aspenström,
Andeŕs S. Byström
Publication year - 1991
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.11.1.213
Subject(s) - saccharomyces cerevisiae , biology , actin , mutant , gene , yeast , microbiology and biotechnology , plasmid , genetics
Recently it was demonstrated that beta-actin can be produced in Saccharomyces cerevisiae by using the expression plasmid pY beta actin (R. Karlsson, Gene 68:249-258, 1988), and several site-specific mutants are now being produced in a protein engineering study. To establish a system with which recombinant actin mutants can be tested in vivo and thus enable a correlation to be made with functional effects observed in vitro, a yeast strain lacking endogenous yeast actin and expressing exclusively beta-actin was constructed. This strain is viable but has an altered morphology and a slow-growth phenotype and is temperature sensitive to the point of lethality at 37 degrees C.