Open AccessDifferential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus.
Author(s) -
Judith A. Potashkin,
R J Derby,
David L. Spector
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.7.3524
Subject(s) - nucleolus , biology , immunoelectron microscopy , cajal body , cell nucleus , microbiology and biotechnology , ribonucleoprotein , nucleus , yeast , schizosaccharomyces pombe , small nuclear ribonucleoprotein , spindle pole body , nuclear protein , saccharomyces cerevisiae , rna , cell , biochemistry , rna splicing , genetics , cell division , gene , spindle apparatus , antibody , transcription factor
The yeast cell nucleus has previously been shown to be divided into two regions by a variety of microscopic approaches. We used antibodies specific for the 2,2,7-trimethylguanosine cap structure of small nuclear ribonucleic acids (snRNAs) and for a protein component of small nuclear ribonucleoprotein particles to identify the distribution of small nuclear ribonucleoprotein particles within the yeast cell nucleus. These studies were performed with the fission yeast Schizosaccharomyces pombe and the budding yeast Saccharomyces cerevisiae. By using immunofluorescence microscopy and immunoelectron microscopy, most of the abundant snRNAs were localized to the portion of the nucleus which has heretofore been referred to as the nucleolus. This distribution of snRNAs is different from that found in mammalian cells and suggests that the nucleolar portion of the yeast nucleus contains functional domains in addition to those associated with RNA polymerase I activity.