Mutational mapping of RAS-responsive domains of the Saccharomyces cerevisiae adenylyl cyclase. | Zendy

John Colicelli | Zendy; Jeffrey Field | Zendy; Roymarie Ballester | Zendy; Nicholas Chester | Zendy; Dallan Young | Zendy; Michael Wigler | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Mutational mapping of RAS-responsive domains of the Saccharomyces cerevisiae adenylyl cyclase.

Author(s) -

John Colicelli,

Jeffrey Field,

Roymarie Ballester,

Nicholas Chester,

Dallan Young,

Michael Wigler

Publication year - 1990

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.10.6.2539

Subject(s) - adenylyl cyclase , biology , saccharomyces cerevisiae , adcy9 , adcy10 , gs alpha subunit , genetics , camp dependent pathway , mutation , adcy3 , gene , synthetic lethality , microbiology and biotechnology , mutant , signal transduction

Large deletion and small insertion mutations in the adenylyl cyclase gene of Saccharomyces cerevisiae were used to map regions required for activation by RAS protein in vitro. The amino-terminal 605 amino acids were found to be dispensable for responsiveness to RAS protein. All other deletions in adenylyl cyclase destroyed its ability to respond to RAS. Small insertion mutations within the leucine-rich repeat region also prevented RAS responsiveness, while other insertions did not.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research