Open AccessIdentification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals.
Author(s) -
Xinmin Cao,
Raymond A. Koski,
Andrea Gashler,
M. A. McKiernan,
Charles F. Morris,
Robert A. Gaffney,
Robert J. Hay,
Vikas P. Sukhatme
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.5.1931
Subject(s) - biology , zinc finger , microbiology and biotechnology , complementary dna , gene product , immunocytochemistry , gene , binding protein , nuclear protein , gene expression , biochemistry , transcription factor , endocrinology
Egr-1 is an immediate-early response gene induced by diverse signals that initiate growth and differentiation. Its cDNA sequence predicts a protein with zinc fingers. We have generated an antiserum to the Egr-1 gene product and identified it as an 80-kilodalton short-lived protein in serum-stimulated mouse fibroblasts. The rat Egr-1 product has also been identified in nerve growth factor-induced PC12 cells. In addition, we show by cell fractionation and immunocytochemistry that the Egr-1 protein is located in the nucleus. We also demonstrate that it is phosphorylated. In vitro-generated Egr-1 protein binds with high affinity to the sequence CGGC in a zinc-dependent manner.