Identification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals. | Zendy

Xing Cao | Zendy; Raymond A. Koski | Zendy; Andrea Gashler | Zendy; M. A. McKiernan | Zendy; Charles F. Morris | Zendy; Robert A. Gaffney | Zendy; R. Hay | Zendy; Vikas P. Sukhatme | Zendy

Open Access

Identification and characterization of the Egr-1 gene product, a DNA-binding zinc finger protein induced by differentiation and growth signals.

Author(s) -

Xing Cao,

Raymond A. Koski,

Andrea Gashler,

M. A. McKiernan,

Charles F. Morris,

Robert A. Gaffney,

R. Hay,

Vikas P. Sukhatme

Publication year - 1990

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.10.5.1931

Subject(s) - biology , zinc finger , microbiology and biotechnology , complementary dna , gene product , immunocytochemistry , gene , binding protein , nuclear protein , gene expression , biochemistry , transcription factor , endocrinology

Egr-1 is an immediate-early response gene induced by diverse signals that initiate growth and differentiation. Its cDNA sequence predicts a protein with zinc fingers. We have generated an antiserum to the Egr-1 gene product and identified it as an 80-kilodalton short-lived protein in serum-stimulated mouse fibroblasts. The rat Egr-1 product has also been identified in nerve growth factor-induced PC12 cells. In addition, we show by cell fractionation and immunocytochemistry that the Egr-1 protein is located in the nucleus. We also demonstrate that it is phosphorylated. In vitro-generated Egr-1 protein binds with high affinity to the sequence CGGC in a zinc-dependent manner.

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