Open AccessIsolation of recessive (mediator-) revertants from NIH 3T3 cells transformed with a c-H-ras oncogene.
Author(s) -
Hisafumi Yamada,
T Omata-Yamada,
N Wakabayashi-Ito,
S G Carter,
Péter Lengyel
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.4.1822
Subject(s) - biology , oncogene , complementation , mediator , 3t3 cells , oncogene proteins , gene , microbiology and biotechnology , mutation , genetics , phenotype , transfection , regulation of gene expression , cell cycle
We have generated two serum- and anchorage-dependent revertants from NIH 3T3 cells transformed with multiple copies of the human c-H-ras oncogene. In both revertants, the c-H-ras oncogene was fully expressed. Fusion of either revertant with untransformed cells or of the two revertants with one another resulted in transformed progeny. These results indicated that the two revertants were recessive and in different complementation groups. We believe that in our two revertants some of the genes mediating the transforming activity of the c-H-ras oncogene are defective; we are attempting to identify these mediator genes.