Open AccessCharacterization of four novel ras-like genes expressed in a human teratocarcinoma cell line.
Author(s) -
George Drivas,
A Shih,
Elias Coutavas,
Mark G. Rush,
P D'Eustachio
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.4.1793
Subject(s) - biology , teratocarcinoma , gene , complementary dna , genetics , oligonucleotide , microbiology and biotechnology , cdna library , dna , genomic library , coding region , peptide sequence , cellular differentiation
A mixed-oligonucleotide probe was used to identify four ras-like coding sequences in a human teratocarcinoma cDNA library. Two of these sequences resembled the rho genes, one was closely related to H-, K-, and N-ras, and one shared only the four sequence domains that define the ras gene superfamily. Homologs of the four genes were found in genomic DNA from a variety of mammals and from chicken. The genes were transcriptionally active in a range of human cell types.