Open AccessDifferential utilization of calcitonin gene regulatory DNA sequences in cultured lines of medullary thyroid carcinoma and small-cell lung carcinoma.
Author(s) -
A de Bustros,
R Y Lee,
Debra L Compton,
Tian Yow Tsong,
Stephen B. Baylin,
Barry D. Nelkin
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.4.1773
Subject(s) - biology , calcitonin , thyroid carcinoma , medullary carcinoma , cell culture , carcinoma , gene , cancer research , microbiology and biotechnology , thyroid , dna , gene expression , medicine , endocrinology , genetics
Regulation of expression of the human calcitonin gene was found to differ between two tumor lines of different tissue origin, medullary thyroid carcinoma (TT line) and small-cell lung carcinoma (DMS53 line). Distal 5' DNA elements between -750 and -2000 exhibited a stronger basal activity in DMS53 than in TT cells, whereas proximal DNA sequences between -132 and -252 mediated a dramatic cyclic AMP response in TT but not DMS53 cells.