Open AccessIdentification of Schizosaccharomyces pombe transcription factor PGA4, which binds cooperatively to Saccharomyces cerevisiae GAL4-binding sites.
Author(s) -
Douglas M. Ruden
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.4.1432
Subject(s) - schizosaccharomyces pombe , biology , saccharomyces cerevisiae , schizosaccharomyces , upstream activating sequence , tata binding protein , transcription factor , dna binding site , cyclin dependent kinase 7 , tata box , binding site , dna binding protein , general transcription factor , transcription (linguistics) , transcription factor ii a , genetics , microbiology and biotechnology , promoter , gene , enhancer , gene expression , cell cycle , cyclin dependent kinase 2 , linguistics , philosophy
When the DNA-binding site for the Saccharomyces cerevisiae transcription activator GAL4 is placed upstream of the Schizosaccharomyces pombe ADH1 TATA box, transcription of the ADH1 gene is activated in S. pombe in vivo by an endogenous transcription factor. In vitro studies show that this S. pombe protein, PGA4, binds specifically to DNA containing a GAL4 site and that when two GAL4 sites are present, this protein binds cooperatively. Cooperating binding of PGA4 to DNA is favored if the GAL4 sites are separated by an integral number of turns of the DNA helix.