Open AccessAltered transcriptional activity of c-fos promoter plasmids in v-raf-transformed NIH 3T3 cells.
Author(s) -
Z Siegfried,
Edward B. Ziff
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.11.6073
Subject(s) - biology , plasmid , microbiology and biotechnology , 3t3 cells , threonine , transcription (linguistics) , serine , gene , transcription factor , promoter activity , promoter , kinase , transfection , phosphorylation , gene expression , genetics , linguistics , philosophy
In cells transformed by v-raf, an oncogenic counterpart of the serine/threonine kinase Raf-1, regulatory elements of the c-fos promoter were active under conditions of cell growth or stimulation for which they were inactive in untransformed control cells. This suggests that v-raf transforms by deregulating transcription of early response genes.