Open AccessFunctional analysis of c-Myb protein in T-lymphocytic cell lines shows that it trans-activates the c-myc promoter.
Author(s) -
J. L. Evans,
Terry L. Moore,
W. Michael Kuehl,
T. Bender,
Jenny P.Y. Ting
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.11.5747
Subject(s) - biology , microbiology and biotechnology , myb , gene , gene expression , expression vector , coding region , clone (java method) , reporter gene , genetics , recombinant dna
The function of c-Myb protein was revealed by transfecting an expression vector containing the entire c-Myb protein-coding sequence into the murine CTLL-2 T-cell line. Expressions of high levels of c-Myb protein did not alter the expression of several T-cell markers, c-fos mRNA expression, responses to interleukin-2, and growth characteristics of these cells. Interestingly, expression of the c-myc gene was drastically increased in this clone. Further, the c-myb expression plasmid, but not a frameshift mutant of c-myb, enhanced the expression of a hybrid construct of c-myc promoter linked to a reporter gene by 8- to 14-fold. These results demonstrate a role of c-Myb protein in c-myc gene expression.