Open AccessStructure of germ line immunoglobulin alpha heavy-chain RNA and its location on polysomes.
Author(s) -
Gail Radcliffe,
Yi-Chaung Lin,
Michael Julius,
Kenneth B. Marcu,
Janet Stavnezer
Publication year - 1990
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.10.1.382
Subject(s) - biology , rna , microbiology and biotechnology , polysome , immunoglobulin heavy chain , messenger rna , antibody , immunoglobulin light chain , gene , genetics , ribosome
We describe the structure of the major germ line RNA transcribed from unrearranged immunoglobulin alpha heavy-chain genes in immunoglobulin M-expressing cells of the I.29 mu B-cell lymphoma, a cell line capable of switching to immunoglobulin A expression upon lipopolysaccharide treatment. This germ line alpha RNA has a small open reading frame that does not include the C alpha domain, and this RNA appears to be present on polysomes in I.29 mu cells.