Open AccessCell transformation mediated by chromosomal deoxyribonucleic acid of polyoma virus-transformed cells.
Author(s) -
G Della Valle,
R. G. Fenton,
Claudio Basilico
Publication year - 1981
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.1.5.418
Subject(s) - biology , dna , transfection , microbiology and biotechnology , transformation (genetics) , southern blot , exogenous dna , homologous recombination , in vitro recombination , homologous chromosome , nucleic acid thermodynamics , gene , polyoma virus , genetics , molecular cloning , peptide sequence , base sequence
To study the mechanism of deoxyribonucleic acid (DNA)-mediated gene transfer, normal rat cells were transfected with total cellular DNA extracted from polyoma virus-transformed cells. This resulted in the appearance of the transformed phenotype in 1 X 10(-6) to 3 X 10(-6) of the transfected cells. Transformation was invariably associated with the acquisition of integrated viral DNA sequences characteristic of the donor DNA. This was caused not by the integration of free DNA molecules, but by the transfer of large DNA fragments (10 to 20 kilobases) containing linked cellular and viral sequences. Although Southern blot analysis showed that integration did not appear to occur in a homologous region of the recipient chromosome, the frequency of transformation was rather high when compared with that of purified polyoma DNA, perhaps due to "position" effects or to the high efficiency of recombination of large DNA fragments.
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