Sam50 Functions in Mitochondrial Intermembrane Space Bridging and Biogenesis of Respiratory Complexes | Zendy

Christine Ott | Zendy; Katharina Roß | Zendy; Sebastian Straub | Zendy; Bernd Thiede | Zendy; Monika Götz | Zendy; Christian Goosmann | Zendy; Markus Krischke | Zendy; Martin J. Mueller | Zendy; Georg Krohne | Zendy; Thomas Rudel | Zendy; Vera KozjakPavlovic | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Sam50 Functions in Mitochondrial Intermembrane Space Bridging and Biogenesis of Respiratory Complexes

Author(s) -

Christine Ott,

Katharina Roß,

Sebastian Straub,

Bernd Thiede,

Monika Götz,

Christian Goosmann,

Markus Krischke,

Martin J. Mueller,

Georg Krohne,

Thomas Rudel,

Vera KozjakPavlovic

Publication year - 2012

Publication title -

molecular and cellular biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 2.14

H-Index - 327

eISSN - 1067-8824

pISSN - 0270-7306

DOI - 10.1128/mcb.06388-11

Subject(s) - biology , intermembrane space , mitochondrial intermembrane space , microbiology and biotechnology , mitochondrion , inner mitochondrial membrane , biogenesis , inner membrane , protein targeting , translocase of the outer membrane , translocase of the inner membrane , membrane protein , bacterial outer membrane , biochemistry , membrane , mitochondrial membrane transport protein , gene , escherichia coli

Mitochondria possess an outer membrane (OMM) and an inner membrane (IMM), which folds into invaginations called cristae. Lipid composition, membrane potential, and proteins in the IMM influence organization of cristae. Here we show an essential role of the OMM protein Sam50 in the maintenance of the structure of cristae. Sam50 is a part of the sorting and assembly machinery (SAM) necessary for the assembly of β-barrel proteins in the OMM. We provide evidence that the SAM components exist in a large protein complex together with the IMM proteins mitofilin and CHCHD3, which we term them itochondriali ntermembrane spaceb ridging (MIB) complex. Interactions between OMM and IMM components of the MIB complex are crucial for the preservation of cristae. After destabilization of the MIB complex, we observed deficiency in the assembly of respiratory chain complexes. Long-term depletion of Sam50 influences the amounts of proteins from all large respiratory complexes that contain mitochondrially encoded subunits, pointing to a connection between the structural integrity of cristae, assembly of respiratory complexes, and/or the maintenance of mitochondrial DNA (mtDNA).

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research