Open AccessAn NK and T Cell Enhancer Lies 280 Kilobase Pairs 3′ to the Gata3 Structural Gene
Author(s) -
Sakie Hosoya-Ohmura,
Yu Hsuan Lin,
Mary S. Herrmann,
Takashi Kuroha,
Arvind Rao,
Takashi Moriguchi,
Kim Chew Lim,
Takamitsu Hosoya,
James Douglas Engel
Publication year - 2011
Publication title -
molecular and cellular biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.14
H-Index - 327
eISSN - 1067-8824
pISSN - 0270-7306
DOI - 10.1128/mcb.05065-11
Subject(s) - biology , enhancer , gata3 , transcription factor , promoter , gene , transgene , microbiology and biotechnology , genetics , gene expression
Transcription factor GATA-3 is vital for multiple stages of T cell and natural killer (NK) cell development, and yet the factors that directly regulateGata3 transcription during hematopoiesis are only marginally defined. Here, we show that neither of theGata3 promoters, previously implicated in its tissue-specific regulation, is alone capable of directingGata3 transcription in T lymphocytes. In contrast, by surveying large swaths of DNA surrounding theGata3 locus, we located acis element that can recapitulate aspects of theGata3 -dependent T cell regulatory programin vivo . This element, located 280 kbp 3′ to the structural gene, directs both T cell- and NK cell-specific transcriptionin vivo but harbors no other tissue activity. This novel, distant element regulates multiple major developmental stages that require GATA-3 activity.